Composite

Part:BBa_K5048070

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)


T7 promoter-LAP-6xHis

LAP protein with a 6xHis tag on the C-terminus. Used to test the expression of LAP.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 51
    Illegal PstI site found at 1520
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1520
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2563
    Illegal BglII site found at 2661
    Illegal XhoI site found at 2714
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 51
    Illegal PstI site found at 1520
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 51
    Illegal PstI site found at 1520
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 2035
    Illegal AgeI site found at 401
    Illegal AgeI site found at 2057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2383


Design

We chose the T7 promoter to ensure the efficiency of the expression of LAP.


Source

See our parts: BBa_I719005, BBa_K5048058


Experiments

See more in our parts: BBa_K5048058


[edit]
Categories
Parameters
None